Abstract

Contrary to what we all learned in physics class, there is in principle no limit to the spatial resolution that can be achieved with a light microscope. The trick is spatially structured illumination light combined with a nonlinear fluorescent response in the specimen. Such illumination encodes high-resolution information in the form of observable low-resolution moiré fringes. Straightforward computational processing can extract the new information and produce an extended-resolution reconstruction.

Reference: Gustafsson, M.G. Nonlinear structured-illumination microscopy: wide-field fluorescence imaging with theoretically unlimited resolution. Proc Natl Acad Sci U S A 102, 13081-13086 (2005).

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