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Daniel Côté, Ph.D.
Assistant Professor
Departement de Physique
Centre de Recherche Université Laval Robert Giffard
2601 Ch. de la Canardiere, F-6500
Québec QC G1J2G3
Canada

Studies of biological processes such as disease progression and response to therapy call for live imaging methods that allow continuous observation without terminating the study subject for histological tissue processing.  Among all current imaging modalities, optical microscopy is the only method capable of probing live tissue with cellular and subcellular resolution.  We describe a video-rate, multimodality imaging system that is designed specifically for live animal imaging.  In vivo depth-sectioned, high-resolution images are obtained using confocal and nonlinear optical techniques that extract structural, functional, and molecular information by combining multiple contrast mechanisms, including fluorescence (single- and two-photon excitation), back scattering, second harmonic generation and coherent anti-Stokes Raman scattering (CARS).   In order to avoid movement artifacts due to animal motion, real-time movement correction is demonstrated. Applications that will be discussed include volume reconstruction of cancer cells in bone marrow cavities, monitoring of stem cell homing to bone marrow, immune response monitoring, and myelin imaging in brain explants and spinal cord.  A study of multiple sclerosis via an animal model and CARS imaging will be presented.