Structured Illumination Microscopy

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X-Ray Serial and Single-Molecule Diffraction Imaging

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Tabletop X-Ray Laser for Cellular Imaging

James Dunn
Franz Weber

This project focuses on the development of a practical x-ray laser source (compact enough that it may fit on a tabletop) – which operates at a frequency within the water-window, in which maximum image contrast between protein and water can be achieved.  We expect that tabletop x-ray lasers will provide unique cellular imaging opportunities for non-synchrotron flash and x-ray radiography.

Imaging Applications of Coherent Anti-Stokes Raman Scattering (CARS)

James Chan
Chris Talley
Steve Lane
Thomas Huser
Doug Taylor
Ted Zwerdling
John Rutledge

Coherent Anti-Stokes Raman Scattering (CARS) is a relatively new laser-based optical method that can identify biomolecules in living cells by detecting the inherent vibrational modes that characterize their chemical structure. As a result, CARS can be used to image cells based on these inherent signals without the need for exogenous labeling, such as fluorescent antibodies. CBST researchers are adapting this technology for use in bioimaging, microscopy, and spectroscopy to investigate the dynamics of label-free cells undergoing different cellular processes.